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.As methods for the determination of DNA sequence improved, it became convenient to search those sequences for potential restriction enzyme cleavage sites since that would permit the facile further manipulation of specific fragments. The algorithm that detects open reading frames ORFs defines them as maximal length segments of DNA from start codons to stop codons in the same reading frame, assuming bacterial sequences and codons.
However, they merely found new utility by then serving as diagnostic reagents to show that DNA constructs had been made correctly.
Abstract NEBcutter, version 1. The program calculates the positions of all restriction enzyme sites noting those that might potentially be blocked by overlapping methylation and finds the ORFs in the sequence.
From the initial display there are many options to go further, including custom digestion with enzymes of your choice and various displays of the digest.