Jumpstarts. ▽. Home > Books > Koss' Diagnostic Cytology and Its Histopathologic Bases. Koss' Diagnostic Cytology and Its Histopathologic Bases View PDF. Request PDF on ResearchGate | On Jul 1, , Jeffrey F. Krane and others published Koss' Diagnostic Cytology and Its Histopathologic Bases, Fifth Edition. Koss' Diagnostic Cytology And Its Histopathologic Bases 2 vol. set-LWW (). pdf - Ebook download as PDF File .pdf), Text File .txt) or read book online.
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Father of cytopathology Dr George Papanicolaou. ▫ The Third Era – consolidation. Dr Leopold Koss Diagnostic Cytology. ▫ The Fourth Era – The Bethesda. Koss' Diagnostic Cytology and Its Histopathologic Bases, Fifth Edition. Editor: Leopold G. Koss, M.D., Dr.h.c., Hon. F.R.C. Pathol (UK). Coeditor: Myron R. Get this from a library! Koss' diagnostic cytology and its histopathologic bases. [ Leopold G Koss; Myron R Melamed;].
Cells dry rapidly once they are spread out on a glass slide.
Cytospray Aerosol Fixative - Immediately spray smear, holding can inches from slide for 12 seconds, air dry, and place in cardboard folders. Bleeding and douching within the previous 24 hours are not contraindications to obtaining specimens.
If a lesion is visualized, a separate smear should be made and labeled appropriately. In selected cases, the clinician may want to submit a vaginal pool smear. This is done by collecting the material with the paddle end of the spatula, spreading it on a second prelabeled glass slide and quickly fixing by either the aerosol spray or ethanol method of fixation. If a two-slide case is submitted in ethanol, the slides must be kept separated by placing a paper clip on the labeled end of the slide or placed into slotted coplin jar.
References Boon ME, M. Principle The usefulness of vaginal hormonal cytology is based on the fact that alterations in the levels and relative concentrations of the gonadal and related adrenal steroids will be reflected in their action on the vaginal epithelium and will be evidenced in the cellular pattern of the cells shed from the vagina.
Proper use of this modality requires a thorough familiarity with the normal cytologic findings in normal females throughout the various physiologic periods of life, an appreciation of the hormonal changes in the various endocrinopathies, and experience in the vaginal cytologic findings in these conditions. Procedure Print the patient's last name and first initial on the frosted end of the glass slide using a No. The speculum must be introduced with no lubricant.
If necessary, normal saline may be used to moisten the speculum. Using the cervical spatula or tongue depressor, lightly scrape the mucosa of the upper third of the vagina and spread the material on the glass slide.
Submit to cytology with an accompanying Cytopathology CRIS Order Requisition Form, properly completed to provide relevant clinical history and a detailed menstrual history. Notes Care must be taken to avoid contamination from the vaginal pool and the ectocervix.
Results The results of cytohormonal studies will be expressed in terms of a Maturation Index MI , and whether the MI is or is not compatible with the patient's age and history. Many factors may make a hormonal evaluation uninterpretable, such as inflammation, infection or the presence of all three squamous cell types. It is also imperative that the LMP be present on the request so that the results can be interpreted.
Principle A synthetic compound with estrogen-like effect, diethylstilbestrol DES was extensively used for prevention of abortions and other complications of pregnancy during the late 's and early 's. About 20 years later, it became apparent that the use of this drug adversely affected the offspring of the patients so treated.
In the female offspring, vaginal and cervical adenosis has resulted. All changes commonly observed in the endocervical epithelium may be observed in adenosis: secretory activity, squamous metaplasia and malignant transformation leading to adenocarcinoma, epidermoid carcinoma or both. Because of these risks, the detection diagnosis and follow-up of patients with adenosis is of considerable practical importance.
Vaginal cytology plays an important role in this respect.
Note: Each vial must be labeled as per the procedure below. Procedure Print the patient's last name, and first initial on the frosted end of the glass slides with the diamond point pen or No. The slides should also be labeled with the additional information codes: CX - for cervical smear ANT - for anterior vaginal wall POST- for posterior vaginal wall RT - for right vaginal wall LT - for left vaginal wall Introduce the speculum without lubricant.
Reference Koss L. Lippincott; , pp.
Principle Cytologic diagnosis of pulmonary carcinoma may be made largely on detection of exfoliated carcinoma cells in sputum or bronchial secretions. The procedure has been mainly used in the diagnostic work-up of symptomatic patients or those with an X-ray abnormality of the chest.
When a pulmonary lesion is suspected, a complete sputum series should be examined. A post-bronchoscopy specimen is considered very valuable, and may be included in the series. Phone Material Needed Wide-mouthed specimen cup with a water-tight lid for each day.
The ml. Procedure I: Routine Give the patient a clean sputum cup the night before and instruct the patient not to use until the following morning before breakfast. Instruct the patient that immediately upon waking, he should sit up and gargle with water, thoroughly rinsing the mouth.
Ask the patient to cough deeply from the diaphragm and expectorate all sputum into the cup. The patient continues the deep coughing and expectorating until a specimen is obtained. Submit the properly labeled specimen immediately to the cytology laboratory with an accompanying Cytopathology CRIS Order Requisition Form completed to include all relevant clinical history.
Notes If sputum cannot be brought immediately to the laboratory, refrigerate. Principle Bronchial specimens for cytologic evaluation augment routine bronchoscopy with biopsy. As the findings at bronchoscopy are usually not predictable beforehand, the operator must be prepared to obtain material by any means which may prove to be the most desirable at the time of examination.
However, if malignancy is the primary concern in a patient who is known or suspected of having TB ,contact Ctopathology regarding special handling of the sample. Principle Bronchoalveolar lavage BAL is a relatively simple procedure in which a high-volume saline lavage of a lung subsegment is done through a bronchoscope in order to obtain cellular and protein constituents from the pulmonary alveolar spaces.
It has been estimated that existing BAL methods can sample more than one million alveoli. In , the same author described and illustrated cancer cells in blood-stained sputum of a patient with lung cancer and in the vomitus of another patient who had gastric cancer [ 5 ].
Lebert — , a French pathologist, collected specimens for cytologic examination from effusions, tracheobronchial secretion, and urine.
He prepared innumerable imprint smears from surgical specimens, including tumors and non-neoplastic lesions. His cytology atlas, published in , contains more than cytologic illustrations. The pictures were taken through the microscope by the newly-invented Deguerre technique [ 6 ]. Paget — prepared smears from needle aspirate of a breast carcinoma in [ 7 ]. Malignant cells were diagnosed in urine in [ 8 ] and in cancer cells were recovered from material passed by the urethra [ 9 ].
By the s, diagnosis of bladder tumors by microscopic examination of urine had become a routine procedure [ 10 ].
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Cytologic examination of sputum was soon introduced as routine laboratory procedure [ 11 ] and microscopic examination of ascitic fluid was employed for diagnosis of peritoneal carcinomatosis [ 12 ]. Malignant cells were recovered from cerebrospinal fluid in [ 13 ] and shortly thereafter gastric washing was introduced [ 14 ]. The s were momentous years in diagnostic cytology. The first monograph of clinical cytology was published in Spain [ 15 ], James Ewing — introduced aspiration cytology in New York City [ 16 ]; and Aurel Babes — , of Rumania, and George Papanicolaou — , of New York City, published papers on detection of uterine cervical cancers by examination of vaginal smears [ 17 , 18 ].
Above all, Ewing and his associates [ 27 — 29 ] popularized needle aspiration cytology and extended its use for deep and superficial lesions Fig. It is of historical interest that while aspiration cytology was used daily as a diagnostic tool in the pathology laboratory of James Ewing at the southwest corner of 68 Street and York Avenue, at the Memorial Hospital for Cancer and Allied Diseases, across the street, at the northeast corner of 68 Street and York Avenue, at the New York Hospital Cornell Medical School, George Papanicolaou and his associates published, in , their epoch-making paper on the diagnostic value of vaginal smears in carcinoma of the uterus [ 30 ].
Papanicolaou with his associates, trainees, and disciples popularized cytology and extended the original technique for collection of specimens and staining of smears to all body sites [ 31 — 40 ].
Koss' Diagnostic Cytology and Its Histopathologic Bases
The first cytology monographs began to appear in the late s [ 41 — 42 ]. In the s, Papanicolaou himself [ 43 ] and others [ 44 — 47 ] published substantial texts on cytologic diagnosis of uterine and other cancers Fig.
Simultaneously, national and international cytology associations were formed and a specialty journal, Acta Cytologica, was inaugurated.
Accurate cytologic diagnosis of upper and lower gastrointestinal cancers was made feasible by introduction of improved lavage and balloon techniques [ 48 — 50 ].JAMA, —, Martinus Nijhoff Publishers, Boston, , pp. During the Functional download koss diagnostic cytology and its histopathologic bases 2 vol. This collector provides a domain of Italian states and quality economies to control your armed campus. Notes The bottle of fixative should be opened and readily accessible before the specimen is obtained.